This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell SP2/0-Ag14 with Balb/c mouse splenocyte immunized with recombinant human p85alpha.
Target:
PI-3
Application Dilute:
WB: 1 µg/mL (chemiluminescence detection system). IP: 1-5 µg/600 µL of cell extract from 5x10cells. ICC: 10 µg/mL
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