5-Bromo-3-indolyl beta-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for beta-galactosidase. 5-Bromo-3-indolyl beta-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl beta-D-galactopyranoside can specifically recognize bacterial beta-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl beta-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of beta-galactosidase activity in mouse embryos or muscle tissues[1][2].
Molecular Weight:
374.18
Purity:
99.73
CAS Number:
[97753-82-7]
Formula:
C14H16BrNO6
Target:
Fluorescent Dye,Glycosidase
Application Notes:
Dye Reagents
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