SYBR Green I chloride is a highly sensitive fluorescent nucleic acid dye that binds specifically to the minor groove of double-stranded DNA or intercalates between base pairs. SYBR Green I chloride exhibits weak fluorescence in the unbound state but emits bright fluorescence upon binding, and it preferentially binds to large-fragment DNA and DNA with high G+C content. SYBR Green I chloride is suitable for real-time PCR technology, its fluorescence intensity correlates with the amount and size of amplification products, enabling accurate quantification of gene expression and discrimination of amplicons via melting curve analysis without additional post-processing. SYBR Green I chloride is widely used in preclinical in vitro nucleic acid detection[1][2].
Molecular Weight:
545.18
Purity:
98.09
CAS Number:
[2225748-05-8]
Formula:
C32H37ClN4S
Target:
DNA/RNA Synthesis,Fluorescent Dye
Application Notes:
MCE Product type: Dye Reagents
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