SYTO9 (solution) is a green fluorescent nucleic acid dye (Ex=485 nm, Em=498 nm (DNA)/501 nm (RNA)). SYTO9 can penetrate the intact cell membrane of viable bacteria. When the cell membrane is damaged (cell death), its rate of entry into cells increases, and it competes with propidium iodide (PI) (HY-D0815), another membrane-impermeable dye, for nucleic acid binding sites. SYTO9 is widely used in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology for bacterial counting, viability assessment, and cell imaging[1][2][3].Solvent and concentration: DMSO: 5 mM
Molecular Weight:
460.57
CAS Number:
[854647-88-4]
Formula:
C25H20N2O3S2
Target:
Fluorescent Dye
Application Notes:
MCE Product type: Dye Reagents
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