7alpha-Hydroxycholesterol is a cholesterol oxide and can serve as a biomarker for oxidative stress and lipid peroxidation. 7alpha-Hydroxycholesterol has cytotoxic and pro-inflammatory activities. 7alpha-Hydroxycholesterol can also inhibit sterol synthesis and reduce the activity of HMG-CoA reductase. 7alpha-Hydroxycholesterol can be used in the research of diseases such as diabetes and atherosclerosis[1][2][3][4][5].