2'-O,4'-C-Methyleneguanosine (LNA-G) is a reverse guanine analog, where LNA (locked nucleic acid) is a nucleic acid analog. LNA modification can be widely used in various fields, such as effective binding affinity with complementary sequences and stronger nuclease resistance than natural nucleotides, providing great potential for application in disease diagnosis and research. 2-O,4-C-Methyleneguanosine is a substrate for KOD DNA polymerase, which incorporates LNA-G nucleotides into growing DNA strands, including consecutive incorporations , to generate full-length extension products[1].
