Monosan Trypsin Pretreatment Kit, 155 ml (125 ml Buffer 30 ml Enzym)

Product Overview

• Article Name: Monosan Trypsin Pretreatment Kit, 155 ml (125 ml Buffer 30 ml Enzym)
• Biozol Catalog Number: MON-APP157
• Supplier Catalog Number: MON-APP157
• Alternative Catalog Number: MON-APP157
• Manufacturer: Monosan
• Category: Kits/Assays
• Application: IF, IHC-Fr, IHC-P

Product Description

Immunohistochemical staining procedures consist of sequential incubation steps with blocking solutions, antibodies and secondary reagents, enzymes and chromogenic substrates carried out on tissue sections. These tissue sections are mostly prepared out of formalin-fixed paraffin-embedded tissue blocks. Cellular structures are very effectively stabilised by formalin fixation which results in optimal morphological preservation of the sample. On the other hand the formalin fixation leads to strong cross-links between proteins. This means that epitopes of antigens are being masked and often are no longer accessible for primary antibodies. In order to enable primary antibodies to bind to antigens the epitopes have to be recovered. Enzymatic digestion with proteolytic enzymes (PIER) restores structures of the epitopes making them more accessible to specific antibodies. Heat induced epitope retrieval (HIER) in buffer solutions of different compositions and pH-values is another way of recovering epitopes. The primary antibody used determines the appropriate method.