Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody, IgG1, Clone: [CDH16/1071], Mouse, Monoclonal

Product Overview

• Article Name: Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody, IgG1, Clone: [CDH16/1071], Mouse, Monoclonal
• Biozol Catalog Number: NBT-1014-MSM1-P0
• Supplier Catalog Number: 1014-MSM1-P0
• Alternative Catalog Number: NBT-1014-MSM1-P0-20,NBT-1014-MSM1-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IHC, WB
• Species Reactivity: Canine, Human, Mouse, Rabbit, Rat
• Immunogen: Recombinant full-length human CDH16 protein
• Alternative Names: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Product Description

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [CDH16/1071]
• Molecular Weight: 130kDa
• Isotype: IgG1
• NCBI: 1014
• UniProt: O75309
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Flow Cytometry (1-2ug/million cells),Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue

Images

Formalin-fixed,

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded Rat Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded Mouse Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Western Blot Analysis of human kidney tissue lysate using KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Formalin-fixed, paraffin-embedded human Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).

Flow Cytometric Analysis of human HEK293 cells using KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).

SDS-PAGE Analysis KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Kidney stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF640R (Purple)