Synthetic peptide corresponding to p21 residues within aa1-100 of p21 was used as an immunogen
Alternative Names:
Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, PIC1, SDI1, SLC12A9, p21Cip1/Waf1, Wild type p53 activated fragment 1 (WAF1)
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (2-4ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
Formalin fixed paraffin embedded human colon carcinoma stained with p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CIP1/4377R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin fixed paraffin embedded human lung carcinoma stained with p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).
Western Blot Analysis of HeLa cell lysate using p21 Rabbit Recombinant Monoclonal Antibody (CIP1/4377R).
Analysis of Protein Array containing more than 19,000 full-length human proteins using p21-Monospecific Rabbit Recombinant Monoclonal Antibody (CIP1/4377R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of Human Kidney, Human Skin and HepG2 lysates using WAF1 Recombinant Rabbit Monoclonal Antibody (CIP1/4377R).
SDS-PAGE Analysis of Purified p21 Recombinant Rabbit Monoclonal Antibody (CIP1/4377R).Confirmation of Purity and Integrity of Antibody.
Western blot analysis of human HepG2 tissue lysate using WAF1 Recombinant Rabbit Monoclonal Antibody (CIP1/4377R).
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