This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Formalin-fixed, paraffin-e
Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).
Flow Cytometry of human p27 on Jurkat cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled p27 Mouse Monoclonal Antibody (KIP1/769).
Formalin-fixed, paraffin-embedded rat Colon Carcinoma stained with p27 Mouse Monoclonal Antibody (KIP1/769).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeling p27 with p27 Mouse Monoclonal Antibody (KIP1/769) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640
SDS-PAGE Analysis Purified p27 Mouse Monoclonal Antibody (KIP1/769). Confirmation of Purity and Integrity of Antibody.
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