p16INK4a is a tumor suppressor protein. It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia. Expression of p16INK4a (p16 positive) is highly correlated with human papilloma virus (HPV) infection in head and neck squamous cell carcinomas (HNSCC). p16 status is an important prognostic indicator in HNSCC and the p16 positive/HPV16 negative group is likely a distinct subgroup lacking any HPV genotype.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
Western blot analysis of HepG2 cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
SDS-PAGE Analysis of Purified P16INK4a Recombinant Rabbit Monoclonal (CDKN2A/7081R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HeLa cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
Western Blot Analysis of HeLa cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
Western Blot Analysis of HEK293T cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/7081R).
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