BCK, Brain creatine kinase, Ckb, Creatine kinase B chain, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine phosphokinase BB, Epididymis luminal protein 211, Epididymis secretory protein Li 29, HEL 211
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), ,Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for
Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Formalin-fixed, paraffin-embedded human kidney stained with CKBB Mou
Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
SDS-PAGE Analysis of Purified CKBB Mouse Monoclonal Antibody (CKBB/6565). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of HEK-293 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Western blot analysis of Y79 cell lysate using CKBB Mouse Monoclonal Antibody (CKBB/6565).
Formalin-fixed, paraffin-embedded human kidney stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).
Formalin-fixed, paraffin-embedded human brain stained with CKBB Mouse Monoclonal Antibody (CKBB/6565).
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