Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, (TM) and A°a, are associated through disulfide bonds. The A°a subunit (also called ApoJ(TM)) corresponds to residues 23-227. The (TM) subunit (also called ApoJA°a) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences A°a-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (2-4ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
Western Blot Analysis of human tissue lysate using Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).
Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of human Tonsil tissue lysate using Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731).
SDS-PAGE Analysis of Purified Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4731). Confirmation of Purity and Integrity of Antibody.
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