Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5
Western Blot Analysis of endometrium using Calponin-1 Mouse Monoclonal Antibody (CALP).
Flow Cytometric Analysis of PFA-fixed K562 cells using Calpon
Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CALP).
Western Blot Analysis of endometrium using Calponin-1 Mouse Monoclonal Antibody (CALP).
Flow Cytometric Analysis of PFA-fixed K562 cells using Calponin-1 Mouse Monoclonal Antibody (CALP ) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence Analysis of PFA-fixed K562 cells labeling Calponin using Calponin-1 Mouse Monoclonal Antibody (CALP ) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
SDS-PAGE Analysis Purified Calponin-1 Mouse Monoclonal Antibody (CALP). Confirmation of Purity and Integrity of Antibody
Western Blot Analysis of Colon lysate using Calponin-1 Mouse Monoclonal Antibody (CALP).
Western Blot Analysis of Colon lysate using Calponin-1 Mouse Monoclonal Antibody (CALP).
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