This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunoprecipitation (1-2ug per 100-500ug of total protein), Western Blot (1-2ug/ml),,Optimal dilution for a specific application should be determined.
Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCR
Flow Cytometric Analysis of PFA-fixed HeLacells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunofluorescence Analysis of HeLacells stained using ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
SDS-PAGE Analysis. Purified ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of PFA-fixed U87cells. ATF2 Mouse Monoclonal Antibody (PCRP-ATF2-1B4) followed bygoat anti-mouse IgG-CF488 (blue), unstained cells (red).
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