Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot of Y79, HeLa and HepG
Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Formalin-fixed, paraffin-embedded human Cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).
Western Blot of Y79, HeLa and HepG2 cell lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).
