This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Flow Cytometric Analysis of human trypsinized MCF-7 cel
Immunofluorescent staining of MeOH-fixed MCF-7 cells with HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Flow Cytometric Analysis of human trypsinized MCF-7 cells. HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
SDS-PAGE Analysis Purified HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of human trypsinized SK-BR3 cells. HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDOOs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDOOs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunofluorescent staining of PFA-fixed MCF-7 cells with HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451), followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
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