Recombinant fragment (around aa129-312) of human ESR1 protein (exact sequence is proprietary)
Alternative Names:
Estrogen Receptor alpha delta 4*5,6,7*/654 isoform, Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1
This monoclonal antibody is specific to estrogen receptor alpha (ER alpha) and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunofluorescence (1-2ug/ml), Flow Cytometry (1-2ug/million cells),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).
Flow Cytometric Analysis of MCF-7 cells using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescence staining of MCF-7 cells using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) followed by goat anti-Mouse IgG-CF488 (green). Membrane stained with Phalloidin (red).
SDS-PAGE Analysis Purified Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557). Confirmation of Integrity and Purity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDis) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDis) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of T-47D lysate using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).
Western Blot Analysis of MCF-7 lysate using Estrogen Receptor alpha Mouse Monoclonal Antibody (ESR1/3557).
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