Blood Group Antigen Lewis B Antibody, IgG1, Clone: [2-25LE, same as LWB01], Mouse, Monoclonal

Product Overview

• Article Name: Blood Group Antigen Lewis B Antibody, IgG1, Clone: [2-25LE, same as LWB01], Mouse, Monoclonal
• Biozol Catalog Number: NBT-2525-MSM1-P1ABX
• Supplier Catalog Number: 2525-MSM1-P1ABX
• Alternative Catalog Number: NBT-2525-MSM1-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC
• Species Reactivity: Guinea pig, Human
• Immunogen: Mucin isolated from a human ovarian cyst fluid
• Alternative Names: Blood group Lewis alpha-4-fucosyltransferase, Fucosyltransferase 3, Fucosyltransferase III, FucT-III, FUT3, Galactoside 3(4)-L-fucosyltransferase, gastric mucin, leB, lewis antigen system, lewis B blood group antigen, Lewis FT, major airway glycoprotein

Product Description

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [2-25LE, same as LWB01]
• Molecular Weight: 45kDa
• Isotype: IgG1
• NCBI: 2525
• UniProt: P21217
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for

Images

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (2-25LE).