Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody, Clone: [ASTRO/1974R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody, Clone: [ASTRO/1974R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-2670-RBM4-P0
• Supplier Catalog Number: 2670-RBM4-P0
• Alternative Catalog Number: NBT-2670-RBM4-P0-20,NBT-2670-RBM4-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Bovine, Canine, Gallus, Guinea pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: Recombinant human full-length GFAP protein
• Alternative Names: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Product Description

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ASTRO/1974R]
• Molecular Weight: ~50kDa
• NCBI: 2670
• UniProt: P14136
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells),Immunofluorescence (2-3ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min a

Images

SDS-PAGE Analysis of Purified GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

Flow Cytometric Analysis of T98G cells usi

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

SDS-PAGE Analysis of Purified GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R).

Flow Cytometric Analysis of T98G cells using GFAP Rabbit Recombinant Monoclonal Antibody (ASTRO/1974R) followed by Goat anti-Rabbit IgG-CF488 (Blue), Isotype Control (Red).

Immunofluorescence Analysis of methanol-fixed human cerebral cortex cryosection stained with CF405S Recombinant Rabbit Anti-GFAP (ASTRO/1974R) (blue) and CF647 Monoclonal Mouse Anti-Histone H1 (HH1/957) (magenta).

Western Blot Analysis of human brain tissue lysate using GFAP Recombinant Rabbit Monoclonal Antibody (ASTRO/1974R).

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain tissue lysates using GFAP Recombinant Rabbit Monoclonal Antibody (ASTRO/1974R).