IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody, IgG2b, Clone: [IM373], Mouse, Monoclonal

Product Overview

• Article Name: IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody, IgG2b, Clone: [IM373], Mouse, Monoclonal
• Biozol Catalog Number: NBT-3507-MSM4-P0
• Supplier Catalog Number: 3507-MSM4-P0
• Alternative Catalog Number: NBT-3507-MSM4-P0-20,NBT-3507-MSM4-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant full-length heavy chain of human IgM
• Alternative Names: AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

Product Description

Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [IM373]
• Molecular Weight: 50-75kDa
• Isotype: IgG2b
• NCBI: 3507
• UniProt: P01871
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM

Images

Western Blot on Raji cell lysate using IgM Mouse Monoclonal Antibody (IM373).

SDS-PAGE Analysis Purified IgM Mouse Monoclonal Antibody (I

SDS-PAGE Analysis of Purified IgM Mouse Monoclonal Antibody (IM373). Confirmation of Integrity and Purity of Antibody

Western Blot on Raji cell lysate using IgM Mouse Monoclonal Antibody (IM373).

Formalin-fixed, paraffin-embedded human Tonsil stained with IgM Mouse Monoclonal Antibody (IM373). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified IgM Mouse Monoclonal Antibody (IM373). Confirmation of Integrity and Purity of Antibody

Western Blot Analysis of Human Serum lysate using IgM Mouse Monoclonal Antibody (IM373)