Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody, IgG1, Clone: [KRT7/760], Mouse, Monoclonal

Product Overview

• Article Name: Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody, IgG1, Clone: [KRT7/760], Mouse, Monoclonal
• Biozol Catalog Number: NBT-3855-MSM4-P0
• Supplier Catalog Number: 3855-MSM4-P0
• Alternative Catalog Number: NBT-3855-MSM4-P0-20,NBT-3855-MSM4-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant full-length human KRT7 protein
• Alternative Names: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Product Description

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Product Properties

• Clonality: Monoclonal
• Clone Designation: [KRT7/760]
• Molecular Weight: 55kDa
• Isotype: IgG1
• NCBI: 3855
• UniProt: P08729
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM E

Images

Formalin-fi

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/760).

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/760).

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/760).

Immunofluorescent staininf of methanol-fixed HeLa cells. KRT7 Mouse Monoclonal Antibody (KRT7/760) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/760).

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells. Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/760) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).

SDS-PAGE Analysis of Purified KRT7 Mouse Monoclonal Antibody (KRT7/760).Confirmation of Purity and Integrity of Antibody.