Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (0.5-1ug/million cells), ,Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Western blot analysis of
Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Formalin-paraffin human endometrial carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Western blot analysis of human lung tissue lysate using Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Immunofluorescence Analysis of MeOH-fixed MCF-7 cells labeling CK19. Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800) followed by goat anti-mouse IgG-CF488 (cyan). Nuclear counterstain is RedDot (red).
Formalin-fixed, paraffin-embedded rat stomach stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
Formalin-fixed, paraffin-embedded rat lung stained with Cytokeratin 19 Mouse Monoclonal Antibody (KRT19/800).
SDS-PAGE Analysis of Purified KRT19 Mouse Monoclonal Antibody (KRT19/800). Confirmation of Purity and Integrity of Antibody.
* VAT and and shipping costs not included. Errors and price changes excepted
