Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires
Western
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).
Western Blot of HeLa, K562 and 293 cell lysates Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).
Western Blot Analysis of HeLa cell lysate using Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).
Confocal immunofluorescence image of HeLa cells using Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831). Green (CF488) and Reddot is used to label the nuclei Red.
SDS-PAGE Analysis Purified Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831). Confirmation of Integrity and Purity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 Monospecific Mouse Monoclonal Antibody (GAL1/1831).
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