Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (5-10ul/million cells), Immunofluorescence (1:50-1:100), Western Blot (1:100-1:200), Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 min at RT)(Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with peps
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Ab (SPM528).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Ep-CAM Ab (SPM528).
* VAT and and shipping costs not included. Errors and price changes excepted
