Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody, IgG1, Clone: [EGP40/826], Mouse, Monoclonal

Product Overview

• Article Name: Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody, IgG1, Clone: [EGP40/826], Mouse, Monoclonal
• Biozol Catalog Number: NBT-4072-MSM6-P1ABX
• Supplier Catalog Number: 4072-MSM6-P1ABX
• Alternative Catalog Number: NBT-4072-MSM6-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 20-65) from the N-terminus of human TACSTD1/EpCAM protein (exact sequence is proprietary)
• Alternative Names: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Product Description

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [EGP40/826]
• Molecular Weight: 40-43kDa
• Isotype: IgG1
• NCBI: 4072
• UniProt: P16422
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA

Images

Confocal Immunofl

4072-MSM6-P1ABX

4072-MSM6-P1ABX

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with EpCAM Mouse Monoclonal Antibody (EGP40/826).

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (EGP40/826). Confirmation of Purity and Integrity of Antibody.

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Mouse Monoclonal Antibody (EGP40/826) (Green). DyLight 554 Phalloidin labeled F-actin filaments (Red). DAPI stained nuclei (blue).

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Mouse MAb (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

Flow Cytometric Analysis of PFA fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (EGP40/826) followed by Goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).

4072-MSM6-P1ABX

Western Blot Analysis of MCF-7 lysate using EpCAM Mouse Monoclonal Antibody (EGP40/826).