Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody, IgG1, Clone: [EGP40/837], Mouse, Monoclonal

Product Overview

• Article Name: Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody, IgG1, Clone: [EGP40/837], Mouse, Monoclonal
• Biozol Catalog Number: NBT-4072-MSM7-P1ABX
• Supplier Catalog Number: 4072-MSM7-P1ABX
• Alternative Catalog Number: NBT-4072-MSM7-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant human TACSTD1 protein
• Alternative Names: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TACSTD1, TROP1, Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2

Product Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [EGP40/837]
• Molecular Weight: 40-43kDa
• Isotype: IgG1
• NCBI: 4072
• UniProt: P16422
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM E

Images

Formalin-fixed, paraffin

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM Mouse Monoclonal Antibody (EGP40/837).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM Mouse Monoclonal Antibody (EGP40/837).

Formalin-fixed, paraffin-embedded human endometrial cancer stained with EpCAM Mouse Monoclonal Antibody (EGP40/837).

Flow Cytometry of human MCF-7 cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled EpCAM Mouse Monoclonal Antibody (EGP40/837).

Western Blot of HCT116 cell lysate using EpCAM Mouse MAb (EGP40/837).

Immunofluorescence Analysis of MCF-7 cells labeling Ep-CAM with EpCAM Mouse MAb (EGP40/837).followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Mouse Monoclonal Antibody (EGP40/837) (Green). DAPI was used to stain the cell nuclei (blue).

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Mouse MAb (IgG1) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody (EGP40/837). Confirmation of Integrity and Purity of Antibody.

4072-MSM7-P1ABX