The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes), Optimal dilution
Formalin-fixed, paraffin-embedded human Colon C
Formalin-fixed, paraffin-embedded human Colon stained with MCM6 Mouse Monoclonal Antibody (MCM6/2999).
Formalin-fixed, paraffin-embedded human Tonsil stained with MCM6 Mouse Monoclonal Antibody (MCM6/2999).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MCM6 Mouse Monoclonal Antibody (MCM6/2999).
SDS-PAGE Analysis Purified MCM6 Mouse Monoclonal Antibody (MCM6/2999). Confirmation of Integrity and Purity of Antibody.
Analysis of Protein Array containing >19,000 full-length human proteins using MCM6 Mouse Monoclonal Antibody (MCM6/2999) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDis) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDis) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of A-431 lysate using MCM6 Mouse Monoclonal Antibody (MCM6/2999).
Western blot analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney and Guinea pig Kidney tissue lysates using MCM6 Mouse Monoclonal Antibody (MCM6/2999).
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