Recombinant MCM7 (Proliferation Marker) Antibody, Clone: [MCM7/2832R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant MCM7 (Proliferation Marker) Antibody, Clone: [MCM7/2832R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-4176-RBM7-P1
• Supplier Catalog Number: 4176-RBM7-P1
• Alternative Catalog Number: NBT-4176-RBM7-P1-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant human MCM7 protein fragment (aa195-319)
• Alternative Names: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Mini chromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146

Product Description

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [MCM7/2832R]
• Molecular Weight: 88kDa
• NCBI: 4176
• UniProt: P33993
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Images

Formalin-fixed, paraffin-embedded human Lymph Node stained with MCM7 Rabbit Recombinant Monoclonal Antibody (MCM7/2832R).

SDS-PAGE Analysis Purified MCM7 Rabbit Recombinant Monoclonal Antibody (MCM7/2832R). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Rabbit Recombinant Monoclonal Antibody (MCM7/2832R).

Western Blot Analysis of MCF-7, HEK-293, A549, SKBr3, HeP2 lysate using MCM7 Mouse Monoclonal Antibody (MCM7&apos,2832R).

Western Blot Analysis of A-431, A549 and HEK293T lysates using MCM7 Rabbit Recombinant Monoclonal Antibody (MCM7/2832R).

Western Blot Analysis of A549 and HEK293 lysates using MCM7 Rabbit Recombinant Monoclonal Antibody (MCM7/2832R).

Western Blot validation of anti-MCM7 antibody. MCM7 protein expression was compared in wild-type (WT) and MCM7 shRNA Knockdown (KD) HT-1080 cells (30ug of total protein). Loading control is shown below.