Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody, Clone: [MGMT/8364R], Rabbit, Monoclonal
Biozol Catalog Number:
NBT-4255-RBM11-P0
Supplier Catalog Number:
4255-RBM11-P0
Alternative Catalog Number:
NBT-4255-RBM11-P0-20,NBT-4255-RBM11-P0-100
Manufacturer:
NeoBiotechnologies
Host:
Rabbit
Category:
Antikörper
Application:
IHC, WB
Species Reactivity:
Human
Immunogen:
Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)
Alternative Names:
6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossv OO OOlinks at the O6v OO OO alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6v OO OOposition of guanine in DNA (prior to crossv OO OOlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNAv OO OOdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Western Blot Analysis of MCF-7 lysate using MGMT Rabbit Recombinant Monoclonal Antibody (MGMT/8364R).
Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).
Western Blot Analysis of Brain and Pancreas lysates of different lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).
Western Blot Analysis of Human Liver, Human Pancreas, Raji and MCF7 lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8364R).
Formalin-fixed, paraffin-embedded human tonsil stained with MGMT Mouse Monoclonal Antibody (MGMT/8364R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human prostate stained with MGMT Mouse Monoclonal Antibody (MGMT/8364R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using MGMT/8364R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified MGMT Mouse Monoclonal Antibody (MGMT/8364R). Confirmation of Purity and Integrity of Antibody.
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