Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Western Blot Analysis of human
Formalin-fixed, paraffin-embedded human Melanoma stained with Moesin Mouse Monoclonal Antibody (MSN/492).
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Moesin Mouse Monoclonal Antibody (MSN/492).
Western Blot Analysis of human Jurkat cell lysate using Moesin Mouse Monoclonal Antibody (MSN/492).
Formalin-fixed, paraffin-embedded human Placenta stained with Moesin Mouse Monoclonal Antibody (MSN/492).
Immunofluorescent staining of PFA-fixed HeLa cells. Moesin Mouse Monoclonal Antibody (MSN/492), followed by goat anti-mouse IgG-CF488 (green). The nuclear counterstain is RedDot (red).
Flow Cytometric Analysis of K562 cells. Moesin Mouse Monoclonal Antibody (MSN/492) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis Purified Moesin Mouse Monoclonal Antibody (MSN/492). Confirmation of Integrity and Purity of Antibody
Western Blot Analysis of PC-3 cell lysate using Moesin Mouse Monoclonal Antibody (MSN/492).
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