Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [NCL/902], Mouse, Monoclonal

Product Overview

• Article Name: Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [NCL/902], Mouse, Monoclonal
• Biozol Catalog Number: NBT-4691-MSM1-P1ABX
• Supplier Catalog Number: 4691-MSM1-P1ABX
• Alternative Catalog Number: NBT-4691-MSM1-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant full-length human NCL protein
• Alternative Names: NCL, Nucl, Nucleolin, Protein C23

Product Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This Mouse Monoclonal Antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [NCL/902]
• Molecular Weight: 76kDa
• Isotype: IgG1
• NCBI: 4691
• UniProt: P19338
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m

Images

Western blot analysis of A431 cell lysate using

Immunofluorescence Analysis of HeLa cells stained with CF647R-labeled Nucleolin Mouse Monoclonal Antibody (NCL/902) (green).

Flow cytometric analysis of human Nucleolin Ag in 293T cells. Black: cells alone, Gray: Isotype Control, Green: AF488-labeled Nucleolin Mouse Monoclonal Antibody (NCL/902).

Flow cytometric analysis of human Nucleolin antigen on Jurkat cells. Grey: isotype control, green: FITC-labeled, purple: APC-labeled, Turquoise: AF700-labeled Nucleolin Mouse Monoclonal Antibody (NCL/902).

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Nucleolin Mouse Monoclonal Antibody (NCL/902).

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Nucleolin Mouse Monoclonal Antibody (NCL/902).

Western blot analysis of A431 cell lysate using Nucleolin Mouse Monoclonal Antibody (NCL/902).

Formalin-fixed, paraffin-embedded human colon stained with Nucleolin Mouse Monoclonal Antibody (NCL/902).

Immunofluorescence Analysis of HeLa cells stained with CF647R-labeled Nucleolin Mouse Monoclonal Antibody (NCL/902) (green). Nuclei have been counterstained with DAPI (blue).

Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (NCL/902) (green) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with phalloidin-CF640.

Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Mouse Monoclonal Antibody (NCL/902) followed by goat anti-mouse IgG-CF488 (green). Membrane is stained with phalloidin-CF640.

Flow cytometric analysis of human Nucleolin Ag in 293T cells. Black: cells alone, Gray: Isotype Control, Green: AF488-labeled Nucleolin Mouse Monoclonal Antibody (NCL/902).

Formalin-fixed, paraffin-embedded human skin stained with Nucleolin Mouse Monoclonal Antibody (NCL/902).

Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin Mouse Monoclonal Antibody (NCL/902).