Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [364-5], Mouse, Monoclonal
Biozol Catalog Number:
NBT-4691-MSM2-P1ABX
Supplier Catalog Number:
4691-MSM2-P1ABX
Alternative Catalog Number:
NBT-4691-MSM2-P1ABX-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
FC, IF, IHC, WB
Species Reactivity:
Human
Immunogen:
lysate of SU-DHL-1 Nuclei
Alternative Names:
NCL, Nucl, Nucleolin, Protein C23
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).
Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).
Formalin-fixed, paraffin-embedded Rat Kidney stained with
Formalin-fixed, paraffin-embedded human Skin stained with Nucleolin Monoclonal Antibody (364-5).
Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5).
Formalin-fixed, paraffin-embedded Rat Kidney stained with AF488-conjugated BrdU Mouse Monoclonal Antibody (BRD469).
Immunofluorescence Analysis of PFA-fixed MCF-7 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Immunofluorescence Analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
Flow Cytometry of Human Nucleolin Ag on 293T cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled Nucleolin Monoclonal Antibody (364-5).
Immunofluorescence Analysis HeLa cells labeling Nucleolin with Nucleolin Mouse Monoclonal Antibody (364-5) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is DAPI (Blue).
SDS-PAGE Analysis Purified with Nucleolin Monoclonal Antibody (364-5). Confirmation of Integrity and Purity of Antibody
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