Recombinant Nucleolin (Marker of Human Cells) Antibody, Clone: [NCL/7014R], Rabbit, Monoclonal
Biozol Catalog Number:
NBT-4691-RBM4-P1ABX
Supplier Catalog Number:
4691-RBM4-P1ABX
Alternative Catalog Number:
NBT-4691-RBM4-P1ABX-100
Manufacturer:
NeoBiotechnologies
Host:
Rabbit
Category:
Antikörper
Application:
FC, IF, IHC, WB
Species Reactivity:
Human
Immunogen:
Recombinant full-length human NCL protein
Alternative Names:
NCL, Nucl, Nucleolin, Protein C23
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
SDS-PAGE Analysis Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). Confirmation of Integrity and Purity of Antibody.
