Ornithine Decarboxylase-1 (ODC-1) Antibody, IgG1, Clone: [ODC1/485], Mouse, Monoclonal

Product Overview

• Article Name: Ornithine Decarboxylase-1 (ODC-1) Antibody, IgG1, Clone: [ODC1/485], Mouse, Monoclonal
• Biozol Catalog Number: NBT-4953-MSM1-P0
• Supplier Catalog Number: 4953-MSM1-P0
• Alternative Catalog Number: NBT-4953-MSM1-P0-20,NBT-4953-MSM1-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full-length human ODC-1 protein
• Alternative Names: Ornithine decarboxylase structural 1, RNODC

Product Description

Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ODC1/485]
• Molecular Weight: 53kDa.
• Isotype: IgG1
• NCBI: 4953
• UniProt: P11926
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Western Blot (0.5-1ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue

Images

Western Blot Analysis of human placental lysat

Flow cytometric analysis of human ODC1 on PC3 cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled ODC1 Monoclonal Antibody (ODC1/485).

Formalin-fixed, paraffin-embedded rat pancreas stained with ODC1 Mouse Monoclonal Antibody (ODC1/485).

Formalin-fixed, paraffin-embedded mouse pancreas stained with ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Western blot analysis of human placental tissue lysate using ODC-1 Mouse Monoclonal Antibody (ODC1/485).

SDS-PAGE Analysis of Purified ODC-1 Mouse Monoclonal Antibody (ODC1/485). Confirmation of Purity and Integrity of Antibody.

Flow cytometric analysis of human ODC-1 in PC3 cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Confocal immunofluorescence image of LNCaP cells using AF488-labeled ODC-1 Mouse Monoclonal Antibody (ODC1/485) (green). Nuclei are counterstained with DAPI (blue).

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Formalin-fixed, paraffin-embedded human testicular carcinoma stained with ODC-1 Mouse Monoclonal Antibody (ODC1/485).

Western blot analysis of PC3 cell lysate using ODC-1 Mouse Monoclonal Antibody (ODC1/485).