PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PC10], Mouse, Monoclonal
Catalog Number:
NBT-5111-MSM1-P1ABX
- Images (10)
| Article Name: | PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PC10], Mouse, Monoclonal |
| Biozol Catalog Number: | NBT-5111-MSM1-P1ABX |
| Supplier Catalog Number: | 5111-MSM1-P1ABX |
| Alternative Catalog Number: | NBT-5111-MSM1-P1ABX-100 |
| Manufacturer: | NeoBiotechnologies |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC, IHC, WB |
| Species Reactivity: | Drosophila, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Yeast, Zebrafish |
| Immunogen: | Rat PCNA/protein A fusion protein |
| Alternative Names: | Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein |
| Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. |
| Clonality: | Monoclonal |
| Clone Designation: | [PC10] |
| Molecular Weight: | 36kDa |
| Isotype: | IgG2a |
| NCBI: | 5111 |
| UniProt: | P12004 |
| Form: | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Antibody Type: | Monoclonal Antibody |
| Application Notes: | Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with |
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Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Mouse Monoclonal Antibody (PC10). |
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SDS-PAGE Analysis of Purified PCNA Mouse Monoclonal Antibody (PC10). |
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Flow Cytometric Analysis of A549 cells using PCNA Mouse Monoclonal Antibody (PC10) fol |
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Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Mouse Monoclonal Antibody (PC10). |
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SDS-PAGE Analysis of Purified PCNA Mouse Monoclonal Antibody (PC10). |
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Flow Cytometric Analysis of A549 cells using PCNA Mouse Monoclonal Antibody (PC10) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red). |
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5111-MSM1-P1ABX |
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Western Blot Analysis of Raji lysate using PCNA Mouse Monoclonal Antibody (PC10). |
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Western Blot Analysis of A-431, 293T, Hep G2, MCF7 and NIH/3T3 cell lysates using PCNA Mouse Monoclonal Antibody (PC10). |
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Western Blot Analysis of Human Spleen, Mouse Spleen, Rat Spleen and Ramos lysate using PCNA Mouse Monoclonal Antibody (PC10). |
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