Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Alternative Names:
DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of HCT116, Jurkat, K-562 and Raji lysates using RNA Poll II Recombinant Rabbit Monoclonal Antibody (POLR2A/9089R).
SDS-PAGE Analysis of Purified RNA Poll II Recombinant Rabbit Monoclonal (POLR2A/9089R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human breast cancer stained with RNA Poll II Recombinant Rabbit Monoclonal Antibody (POLR2A/9089R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western Blot Analysis of human T47D cell lysate using RNA Poll II Recombinant Rabbit Monoclonal Antibody (POLR2A/9089R).
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