Synthetic peptide corresponding to (aa 162-176) of human ACTH (NGAEDESAEAFPLEF)
Alternative Names:
Adrenocorticotropin, alpha, beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH), does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Clonality:
Monoclonal
Clone Designation:
[CLIP/1407]
Molecular Weight:
ACTH is ~5kDa|and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Formalin-fixed, paraffin-embedded human pituitary adenoma stained with ACTH Mouse Monoclonal Antibody (CLIP/1407).
Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH Mouse Monoclonal Antibody (CLIP/1407).
Formalin-fixed, paraffin-embedded human pituitary stained with ACTH Mouse Monoclonal Antibody (CLIP/1407).
SDS-PAGE Analysis of Purified ACTH Mouse Recombinant Monoclonal Antibody (rCLIP/1407). Confirmation of Purity and Integrity of Antibody.
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