OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody, IgG2b, Clone: [3508-10-04 00:00:00], Mouse, Monoclonal

Product Overview

• Article Name: OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody, IgG2b, Clone: [3508-10-04 00:00:00], Mouse, Monoclonal
• Biozol Catalog Number: NBT-5460-MSM1-P0
• Supplier Catalog Number: 5460-MSM1-P0
• Alternative Catalog Number: NBT-5460-MSM1-P0-20,NBT-5460-MSM1-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)
• Alternative Names: OCT3, OCT3A, OCT4, OCT4A, OCT-3/4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, octamer-binding transcription factor-3, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1

Product Description

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells, its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [3508-10-04 00:00:00]
• Molecular Weight: 38.6kDa
• Isotype: IgG2b
• NCBI: 5460
• UniProt: Q01860
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Images

Formalin-fixed, paraffin-embedded human seminoma stained with OCT-4 Mo

Formalin-fixed, paraffin-embedded human seminoma stained with OCT-4 Mouse Monoclonal Antibody (OCT4/3508) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT-4 Mouse Monoclonal Antibody (OCT4/3508) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Western blot analysis of NCCIT cell lysate using OCT-4 Mouse Monoclonal Antibody (OCT4/3508).