Isolated neoplastic cells from T cell lymphoma (2B11), and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26)
Alternative Names:
B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95
Flow Cytometric Analysis of PFA-fixed Jurk
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45 Mouse Monoclonal Antibody (2B11+PD7/26).
Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 Mouse Monoclonal Antibody (2B11+PD7/26) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Western Blot Analysis of human Spleen tissue lysates using CD45 Mouse Monoclonal Antibody (2B11+PD7/26).
Flow Cytometric Analysis of live Jurkat cells unstained (grey) and CD45 Mouse Monoclonal Antibody (2B11+PD7/26) conjugated with CF680 (purple).
Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) and stained with CF488A-labeled CD45 monoclonal antibody (2B11+PD7/26) (green).
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