Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody, Clone: [ACTA2/1614R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody, Clone: [ACTA2/1614R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-59-RBM4-P1ABX
• Supplier Catalog Number: 59-RBM4-P1ABX
• Alternative Catalog Number: NBT-59-RBM4-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: Recombinant full-length human ACTA2 protein
• Alternative Names: ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Product Description

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ACTA2/1614R]
• Molecular Weight: 42kDa
• NCBI: 59
• UniProt: P62736
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Images

Formalin-fixed, paraffin-embedded human uterus stained with Smooth Muscle Actin Recombinant Rabbit Monoclonal (ACTA2/1614R).

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Smooth Muscle Actin Recombinant Rabbit Monoclonal (ACTA2/1614R).

SDS-PAGE Analysis of Purified SMA Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R). Confirmation of Integrity and Purity of Antibody.

Western Blot analysis of Human Kidney tissue lysate using CDH16 Recombinant Rabbit Monoclonal Antibody (CDH16/1532R).

Western Blot Analysis of Mouse 3T3 lysate using ACTA2 Rabbit Recombinant Monoclonal Antibody (ACTA2/1614R).

Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney and Rabbit Kidney tissue lysates using Actin Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R).

Western Blot analysis of Human Kidney tissue lysate using ACTA2 Recombinant Rabbit Monoclonal Antibody (ACTA2/1614R)

Western Blot Analysis of Mouse NIH/3T3 and human kidney tissue lysates using Actin, Smooth Muscle (ACTA2) Rabbit Recombinant Monoclonal Antibody (ACTA2/1614R).

59-RBM4-P1ABX

Western Blot analysis of Human Brain Tissue and A375 lysates using S100B Recombinant Rabbit Monoclonal Antibody (S100B/1706R).

Western Blot analysis of Raji cell lysate using CD79a Recombinant Rabbit Monoclonal Antibody (IGA/1688R).

Western Blot analysis of Human Skin and A431 lysates using Cytokeratin, Acidic (Type I or LMW) Recombinant Rabbit Monoclonal Antibody (KRTL/1577R).

Western Blot analysis of Human Skin and A431 lysates using Cytokeratin, pan Recombinant Rabbit Monoclonal Antibody (KRTH/1576R + KRTL/1577R).