Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Mouse Monoclonal Antibody (SPM587).
Western Blot Analysis of Cyclin D1 in (1) C2C12, (2) HepG2, & (3) NIH3T3 lysate with Cyclin D1 Ab (SPM587).
Flow cytometric analysis of human Cyclin D1 on Jurkat cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).
Flow cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587).
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