A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Alternative Names:
Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), ,Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1
Formalin-fixed, paraffin-embedded human tonsilstained with Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418).
SDS-PAGE Analysis Purified Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HDLM-2 cell lysate using Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/6418).
Western Blot Analysis of Human Skeletal muscle, Human Spleen, Jurkat and Raji lysates using Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/6418).
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