S100-A9, MRP14, calgranulin-B, calprotectin L1H subunit, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14, S100 calcium-binding protein A9 (calgranulin B)
This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffers cells of the liver and Langerhans cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (For staining of formalin-fixed, paraffin-embedded tissues, digest with trypsin at 1mg/ml PBS, 15 min at RT Optimal
SDS-PAGE Analysis of Purified Calprotectin Mouse Monoclonal Antibody (S100A9/1075). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human breast cancer stained with Calprotectin Mouse Monoclonal Antibody (S100A9/1075). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human breast cancer stained with Calprotectin Mouse Monoclonal Antibody (S100A9/1075). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min
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