Calcineurin B homologous protein 2 / HCC Antigen 520 Antibody, IgG2c, Clone: [CPTC-CHP2-1], Mouse, Monoclonal

Product Overview

• Article Name: Calcineurin B homologous protein 2 / HCC Antigen 520 Antibody, IgG2c, Clone: [CPTC-CHP2-1], Mouse, Monoclonal
• Biozol Catalog Number: NBT-63928-MSM1-P0
• Supplier Catalog Number: 63928-MSM1-P0
• Alternative Catalog Number: NBT-63928-MSM1-P0-20,NBT-63928-MSM1-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant human full-length CHP2 protein
• Alternative Names: calcineurin-like EF hand protein 2, calcineurin-like EF-hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520, Hepatocellular carcinoma-associated antigen 520 homolog

Product Description

CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [CPTC-CHP2-1]
• Molecular Weight: 22kDa
• Isotype: IgG2c
• NCBI: 63928
• UniProt: O43745
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Western Blot (1-2ug/ml), Immunofluorescence (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC f

Images

Western Blot Analysis of MCF-7 & PC3 cell lysates using CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1).

Immunofluorescence Analysis of human MCF-7 cells labeling CHP2 with CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1) followed by Goat anti-mouse IgG-CF488 (Green).The nuclear counterstain is Reddot (Red)

SDS-PAGE Analysis of Purified CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1). Confirmation of Purity and Integrity of Antibody.