NECAB1 / N-terminal EF-hand calcium binding protein 1 Antibody, Clone: [NECAB1/7680], Mouse, Monoclonal

Product Overview

• Article Name: NECAB1 / N-terminal EF-hand calcium binding protein 1 Antibody, Clone: [NECAB1/7680], Mouse, Monoclonal
• Biozol Catalog Number: NBT-64168-MSM5-P0
• Supplier Catalog Number: 64168-MSM5-P0
• Alternative Catalog Number: NBT-64168-MSM5-P0-20,NBT-64168-MSM5-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant human NECAB1 protein
• Alternative Names: EF-hand calcium-binding protein 1, EFCBP1, N-terminal EF-hand calcium-binding protein 1, Neuronal calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1

Product Description

Members of the EF-CBP (N-terminal EF-hand calcium binding protein)/NECAB (neuronal calcium-binding protein) family participate in neuronal calcium signaling. EF-CBP1 (N-terminal EF-hand calcium binding protein 1), also known as STIP-1 or neuronal calcium-binding protein 1 (NECAB1), is a 351 amino acid cytoplasmic protein that contains one antibiotic biosynthesis monooxygenase (ABM) domain and 2 EF-hand domains. Expressed in brain, EF-CBP1 interacts with copine 6 and Syntaxin, and exists as two alternatively spliced isoforms. The gene encoding EF-CBP1 maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [NECAB1/7680]
• Molecular Weight: 41kDa
• NCBI: 64168
• UniProt: Q8N987
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio

Images

Western Blot Analysis of Human Brain tissue lysate using NECAB1 Mouse Monoclonal Antibody (NECAB1/7680).

SDS-PAGE Analysis of Purified NECAB1 Mouse Monoclonal Antibody (NECAB1/7680). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human brain stained with NECAB1 Mouse Monoclonal Antibody (NECAB1/7680). Inset: PBS instead of primary antibody, secondary only negative control.

Formalin-fixed, paraffin-embedded human brain stained with NECAB1 Mouse Monoclonal Antibody (NECAB1/7680). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.