BRAF Antibody, IgG1, Clone: [BRAF/1626], Mouse, Monoclonal

Product Overview

• Article Name: BRAF Antibody, IgG1, Clone: [BRAF/1626], Mouse, Monoclonal
• Biozol Catalog Number: NBT-673-MSM5-P1ABX
• Supplier Catalog Number: 673-MSM5-P1ABX
• Alternative Catalog Number: NBT-673-MSM5-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human HMG20B protein (exact sequence is proprietary)
• Alternative Names: BRAF25, BRAF35, BRCA2-associated factor 35, High Mobility Group Protein 20B (HM20B), HMG box-containing protein 20B, HMG domain-containing protein HMGX2, HMG20B, SMARCE1-related protein, SMARCE1r, Sox-like transcriptional factor, SOXL

Product Description

The OOBRAF OOgene encodes a cytoplasmic serine-threonine kinase, which initiates the activation of the mitogen-activated protein kinase (MAPK) signalling pathway. The oncogenic mutations in the kinase region of BRAF gene result in constitutive activation of the MAPK signalling pathway, leading to increased cell proliferation, resistance to apoptosis and tumor progression. The most common of all activating BRAF mutations leads to a substitution of valine (V) to glutamic acid (E) at the position 600 of the amino acid sequence. The OOBRAF OOV600E mutation is an important predictive and prognostic biomarker. The BRAF V600E mutation is detected in approximately 8% of all solid tumours, including 45% of papillary thyroid carcinomas, 40-60% of melanomas, 5-15% of colorectal adenocarcinomas, 35% of serous low grade and borderline ovarian carcinomas, 1-3% of non-small cell lung cancers, and 5-7% of cholangiocarcinomas. OOFurthermore, the BRAF V600E mutation is found in 100% of hairy cell leukaemia, OO54% Erdheim-Chester disease, 38% of Langerhans cell histocytoses OOand 60% of pleomorphic xanthoastrocytomas. OO

Product Properties

• Clonality: Monoclonal
• Clone Designation: [BRAF/1626]
• Molecular Weight: 35kDa (predicted)
• Isotype: IgG1
• NCBI: 673
• UniProt: Q9P0W2
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Images

SDS-PAGE Analysis of Purified BRAF Mouse Monoclonal Antibody (BRAF/1626). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of Human Lung, Small Intestine, Gallbladder, Pancreas tissue lysates using BRAF Mouse Monoclonal Antibody (BRAF/1626).

Formalin-fixed, paraffin-embedded human ovarian cancer stained with BRAF Mouse Monoclonal Antibody (BRAF/1626). Inset: PBS instead of primary antibody, secondary only negative control.