This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinsons disease.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain tissue lysates using PGP9.5 / UchL1 Recombinant Mouse Monoclonal Antibody (rUCHL1/8133).
Western Blot Analysis of human brain tissue lysate using PGP9.5 / UchL1 Recombinant Mouse Monoclonal Antibody (rUCHL1/8133).
Formalin-fixed, paraffin-embedded human cerebellum stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/8133). Inset: PBS instead of primary antibody, secondary only negative control.
SDS-PAGE Analysis of Purified Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/8133). Confirmation of Purity and Integrity of Antibody.
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