Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
Alternative Names:
VIL1, Villin-1, Villin1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed b
Western Blot of A549, HepG2 & HCT116 cell lysates with Villin Monoclonal Antibody (VIL1/1314).
Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1314).
Formalin-fixed, paraffin-embedded human Colon stained with
Western Blot of A549, HepG2 & HCT116 cell lysates with Villin Monoclonal Antibody (VIL1/1314).
Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1314).
Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1314).
SDS-PAGE Analysis of Purified Villin Monoclonal Antibody (VIL1/1314). Confirmation of Purity and Integrity of Antibody.
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