Recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)
Alternative Names:
VIL1, Villin-1, Villin1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 2
SDS-PAGE Analysis Purified Villin Rabbit Recombinant Monoclonal Antibody (VIL1/2310R). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human Small Intestine stained with Villin Rabbit Recombinant Monoclonal Antibody (VIL1/2310R).
Western Blot Analysis of COLO 38 cell lysate using Villin Recombinant Rabbit Monoclonal Antibody (VIL1/2310R).
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